XRCC6 Antibody, FITC conjugated

$210.00$377.00

Cat. No.: 43596 Categories: ,

Description

Aliases

5-deoxyribose-5-phosphate lyase Ku70, 70 kDa subunit of Ku antigen, ATP-dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70 kDa subunit , CTC box-binding factor 75 kDa subunit

Antibody Type

Polyclonal Antibody

Species

human

Uniprot ID

P12956

Immunogen

Recombinant human X-ray repair cross-complementing protein 6 ptotein (2-609AA)

Raised In

Rabbit

Species Reactivity

Human

Tested Applications

ELISA;Not yet tested in other applications.

Background / Function

Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3-5 direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5′-deoxyribose-5-phosphate lyase (5′-dRP lyase), by catalyzing the beta-elimination of the 5′ deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5′-dRP lyase activity allows to ‘clean’ the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.

Isotype

IgG

Conjugate

FITC

Storage Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Caprylic Acid Ammonium Sulfate Precipitation purified

Research

Epigenetics and Nuclear Signaling

Literature

[1]Cloning and characterization of a cDNA that encodes a 70-kDa novel human thyroid autoantigen.Chan J.Y., Lerman M.I., Prabhakar B.S., Isozaki O., Santisteban P., Kuppers R.C., Oates E.L., Notkins A.L., Kohn L.D.J. Biol. Chem. 264:3651-365

Additional information

Size

50μg, 100μg

Certificate of Analysis