Name: RPMI-1640 Medium, GlutaPlus, HEPES, Sodium Pyruvate
SKU: G4535
Availability: InStock

RPMI-1640 Medium, GlutaPlus, HEPES, Sodium Pyruvate

$36.00 – $67.00

Size

500 mL, 1 L (2×500 mL)

RPMI-1640 is a cell culture medium developed by the Roswell Park Memorial Institute (RPMI), and contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, without protein or growth factors. Need to add 5-10% serum or serum-free additives depending on the cell type. This medium was originally designed specifically for lymphocytes culture, now is widely used for various normal cell and cancer cells culture, especially for cells suspension culture, and is one of the most widely used medium.

This product is filtered and sterilized by 0.1 μm filter membrane, pH 7.0-7.4, contains 11.1 mM D-glucose,2.05mML-Alanyl-L-Glutamine,phenol red indicator, HEPES and sodium pyruvate.

L-Alanyl-L-Glutamine is a cell culture additive, replaces the equivalent molar amount of L-glutamine in cell culture medium. Itis very stable in aqueous solution and does not degrade spontaneously. Instead, it is slowly degraded to release glutamine through the peptidase secreted by cells, then absorbed and utilized by cells.

HEPES is an excellent biological buffer with no effect toxic on cells, and the medium with HEPES can maintain a constant pH range for a longer period of time.

Storage and Shipping Conditions

Ship at room temperature; Store away from light at 2-8℃, valid for 12 months.

Note

1. This product has been filtered and sterilized. Attention should be paid to aseptic operation to avoid contamination.

2. For best results, do not freeze and thaw repeatedly.

3. For your safety and health, please wear safety glasses, gloves and protective clothing.

For Research Use Only!

G4535 RPMI-1640 Formula

Components	Concentration		Components	Concentration
Components	mg/L	mM	Components	mg/L	mM
Amino acids			Inorganic salts
Glycine	10	0.133	Calcium nitrate (Ca(NO₃)₂ ₄H₂O)	100	0.4237
L-Arginine hydrochloride	241.86	1.149	Magnesium Sulfate (MgSO₄) (anhydrous)	48.84	0.407
L-Asparagine hydrate	56.82	0.379	Potassium Chloride (KCl)	400	5.33
L-Aspartic Acid	20	0.15	Sodium Bicarbonate (NaHCO₃)	2000	23.8
L-Cystine dihydrochloride	65.2	0.208	Sodium Chloride (NaCl)	5300	90.6
L-GlutamicAcid	20	0.136	Sodium Phosphate monobasic (NaH₂PO₄) (anhydrous)	800	5.63
L-HistidineHCl₂O	20.22	0.097	Other components
L-Hydroxyproline	20	0.153	D-Glucose (Dextrose)	2000	11.11
L-Isoleucine	50	0.382	Sodium pyruvate	110	1.0
L-Leucine	50	0.382	Glutathione (reduced)	1.0	0.003
L-Lysine hydrochloride	40	0.219	HEPES	5958	25
L-Methionine	15	0.101	Phenol Red	4.6	0.013
L-Phenylalanine	15	0.091
L-Proline	20	0.174
L-Serine	30	0.286
L-Threonine	20	0.168
L-Tryptophan	5.0	0.025
L-Tyrosine Disodium Salt Dihydrate	28.83	0.111
L-Valine	20	0.171
L-Alanyl-L-Glutamine	446	2.055
Vitamins
Biotin	0.2	0.00082
Choline chloride	3.0	0.02143
D-Calcium pantothenate	0.25	0.00052
Folic Acid	1.0	0.00227
Niacinamide	1.0	0.00820
Para-Aminobenzoic Acid	1.0	0.00730
Pyridoxine hydrochloride	1.0	0.00485
Riboflavin	0.2	0.00053
Thiamine hydrochloride	1.0	0.00297
Vitamin B12	0.005	0.00000369
i-Inositol	35	0.19444

MAECENAS IACULIS

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ADIPISCING CONVALLIS BULUM

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SKU: G4535 Categories: Culture Media, Mammalian Cell Culture

Description

Components	Concentration		Components	Concentration
Components	mg/L	mM	Components	mg/L	mM
Amino acids			Inorganic salts
Glycine	10	0.133	Calcium nitrate (Ca(NO₃)₂ ₄H₂O)	100	0.4237
L-Arginine hydrochloride	241.86	1.149	Magnesium Sulfate (MgSO₄) (anhydrous)	48.84	0.407
L-Asparagine hydrate	56.82	0.379	Potassium Chloride (KCl)	400	5.33
L-Aspartic Acid	20	0.15	Sodium Bicarbonate (NaHCO₃)	2000	23.8
L-Cystine dihydrochloride	65.2	0.208	Sodium Chloride (NaCl)	5300	90.6
L-GlutamicAcid	20	0.136	Sodium Phosphate monobasic (NaH₂PO₄) (anhydrous)	800	5.63
L-HistidineHCl₂O	20.22	0.097	Other components
L-Hydroxyproline	20	0.153	D-Glucose (Dextrose)	2000	11.11
L-Isoleucine	50	0.382	Sodium pyruvate	110	1.0
L-Leucine	50	0.382	Glutathione (reduced)	1.0	0.003
L-Lysine hydrochloride	40	0.219	HEPES	5958	25
L-Methionine	15	0.101	Phenol Red	4.6	0.013
L-Phenylalanine	15	0.091
L-Proline	20	0.174
L-Serine	30	0.286
L-Threonine	20	0.168
L-Tryptophan	5.0	0.025
L-Tyrosine Disodium Salt Dihydrate	28.83	0.111
L-Valine	20	0.171
L-Alanyl-L-Glutamine	446	2.055
Vitamins
Biotin	0.2	0.00082
Choline chloride	3.0	0.02143
D-Calcium pantothenate	0.25	0.00052
Folic Acid	1.0	0.00227
Niacinamide	1.0	0.00820
Para-Aminobenzoic Acid	1.0	0.00730
Pyridoxine hydrochloride	1.0	0.00485
Riboflavin	0.2	0.00053
Thiamine hydrochloride	1.0	0.00297
Vitamin B12	0.005	0.00000369
i-Inositol	35	0.19444

Manual

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Safety Datasheet

Download Safety Datasheet

Certificate of Analysis

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