SPAM1 Antibody, HRP conjugated

Aliases Hyal-PH20, Hyaluronoglucosaminidase PH-20, Sperm adhesion molecule 1, Sperm surface protein PH-20, HYAL3, PH20, SPAM1 Antibody Type Polyclonal Antibody Species Human Uniprot ID P38567 Immunogen Recombinant human Hyaluronidase PH-20 protein (36-490AA) Raised In Rabbit Species Reactivity Human Tested Applications ELISA;Not yet tested in other applications. Background / Function Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid. Isotype IgG Conjugate HRP Storage Buffer Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 Form Liquid Storage Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. Purity Caprylic Acid Ammonium Sulfate Precipitation purified Literature [1]”Exome sequencing identifies frequent mutation of the SWI/SNF complex gene PBRM1 in renal carcinoma.” Varela I., Tarpey P., Raine K., Huang D., Ong C.K., Stephens P., Davies H., Jones D., Lin M.L., Teague J., Bignell G., Butler A., Cho J., Dalgliesh G.L., Galappaththige D., Greenman C., Hardy C., Jia M. Futreal P.A. Nature 469:539-542(2010). [2]”In vitro mutagenesis of PH-20 hyaluronidase from human sperm.” Arming S., Strobl B., Wechselberger C., Kreil G. Eur. J. Biochem. 247:810-814(1996). [3]”Expression analysis, genomic structure, and mapping to 7q31 of the human sperm adhesion molecule gene SPAM1.” Jones M.H., Davey P.M., Aplin H., Affara N.A. Genomics 29:796-800(1994).