TOP1 Antibody

Conjugate: Non-conjugated. Product Type: Recombinant Antibody. Clonality: Monoclonal. Form: Liquid. Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Clone Number: 6D8. Aliases: DNA topoisomerase 1 (EC 5.99.1.2) (DNA topoisomerase I), TOP1. Immunogen Species: Homo sapiens (Human). UniProtID: P11387. Immunogen: A synthesized peptide derived from human TOP1. Species Reactivity: Human. Tested Applications: ELISA, WB, IHC, FC, IP; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, FC:1:20-1:200, IP:1:200-1:1000. Background: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3′-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5′-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5′-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter. Isotype: Rabbit IgG. Purificaiton Method: Affinity-chromatography. Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Target Names: TOP1.