Akt (Phospho-Tyr326) Antibody

$454.00

Cat. No.: 38689 Categories: ,

Description

Aliases

AKT; AKT1 kinase; C-AKT; EC 2.7.11.1; PKB; PKB-alpha; Protein kinase B; RAC; RAC-PK-alpha; RAC-alpha serine/threonine kinase; kinase Akt1

Antibody Type

Polyclonal Antibody

Uniprot ID

Swiss-Prot#:P31749;
NCBI Gene#:207

Immunogen

Peptide sequence around phosphorylation site of tyrosine 326 (N-D-Y(p)-G-R) derived from Human Akt.

Raised In

Rabbit

Species Reactivity

Human Mouse Rat

Tested Applications

WB Recommended dilution: Western blotting: 1:500~1:3000,

Background / Function

AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. Phosphorylation of PTPN1 at ‘Ser-50’ negatively modulates its phosphatase activity preventing dephosphorylation of the insulin receptor and the attenuation of insulin signaling.

Conjugate

Unconjugated

Storage Buffer

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Modification

Phospho-Tyr326

Additional information

Size

100?l

Certificate of Analysis