Genomic DNA Extraction Kit – Gram(+) Bacteria / Yeast / Fungi

$219.00

Clear
Cat. No.: 10645 Categories: ,

Description

Our Genomic DNA Extraction Kit – Gram(+) Bacteria / Yeast / Fungi kit is specially designed for extracting genomic DNA from Gram positive bacteria, Yeast, and Fungi. Optimized chaotropic salt and detergents are used to extract genomic DNA efficiently by pre-lysis and lysis buffer system. The kit provides a fast and easy way to purify DNA from various Gram-positive bacteria, yeast, fungal tissue and fungi. The method involves preparation of spheroplast, using the enzymes: lysozyme (in bacteria & fungi) or lyticase (in yeast) and the DNA present in the sample is then selectively bound to the spin column provided with the kit. After washing the spin column with the Wash Buffer, the DNA is eluted from the column with a small volume of an elution buffer, TE. The isolated DNA is suitable for PCR and other downstream applications. The supplied kit is suitable for 50 sample preparations.

 

Kit Components:

Items Cat. No. 10645 Storage
Suspension Buffer 15 ml 4°C
Lysis Buffer 25 ml 4°C
Binding Buffer 15 ml 4°C
Wash Buffer (Concentrate) 10 ml 4°C
Lysozyme 0.2 ml -20°C
Lyticase 0.2 ml -20°C
RNase A 0.3ml -20°C
Proteinase K 0.5ml -20°C
Elution Buffer 5 ml 4°C
Spin Columns 50 Columns RT
Collection Tubes (2.0 ml) 50 Tubes RT

Additional information

Size

50 Preps

Certificate of Analysis