ABL1/2 (phospho-Tyr393/439) Antibody

$454.00

Cat. No.: 39043 Categories: ,

Description

Aliases

Abelson murine leukemia viral oncogene homolog 1; c-ABL; p150

Antibody Type

Polyclonal Antibody

Uniprot ID

Swiss-Prot: P00519 P42684
NCBI Protein: NP_005

Immunogen

Peptide sequence around phosphorylation site of tyrosine 393/439 (D-T-Y(p)-T-A) derived from Human ABL1/2.

Raised In

Rabbit

Species Reactivity

Human Mouse

Tested Applications

WB IHC IF Recommended dilution: Predicted MW: 210kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Background / Function

Regulates cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. Localizes to dynamic actin structures, and phosphorylates CRK and CRKL, DOK1, and other proteins controlling cytoskeleton dynamics. Regulates DNA repair potentially by activating the proapoptotic pathway when the DNA damage is too severe to be repaired. Phosphorylates PSMA7 that leads to an inhibition of proteasomal activity and cell cycle transition blocks. Wang, J.Y. et al. (2000) Oncogene 19, 5643-5650. Danial, N.N. et al. (2000) Oncogene 19, 2523-2531. Brasher, B.B. et al. (2000) J. Biol. Chem. 275, 35631-35637. Pluk, H. et al. (2002) Cell 108, 247-259.

Conjugate

Unconjugated

Storage Buffer

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Modification

Phospho-Tyr393/439

Additional information

Size

100?l

Certificate of Analysis