Source protein: Protein A affinity chromatography purified protein from mouse ascites fluid.
Clonality and Host Species: HIS. H8, Mouse
Immunogen and Isotype: HHHHHH (6x His) synthetic peptide conjugated to KLH, IgG2b
Specificity and reactivity: Detects the epitope of 6x His-Tags encoded by many commercially available vectors, regardless of the tag’s location in the fusion protein sequences (reacts with N-terminal, C-terminal or internal 6X His-tags).
Application: Western blot, Dot blot, ELISA, IHC/IS, Western blot (with ECL) 1-2 ng of His-tagged fusion protein.
Available form: 50 µg of lyophilized antibody (lyophilized in 10mM PBS, pH 7.2 with 1% BSA and 0.05% Sodium azide; NaN3)
Working procedure: Western blot (with ECL) 1:1000-3000 dilution incubates for 1 hour at room temperature. For the best results, determine the optimal working antibody dilution for your protein of interest.
Storage: Supplied lyophilized antibodies can be stored at -20ºC for 3 years. Reconstitute the lyophilized antibody with distilled water to 1mg/ml or a desired concentration before use. Reconstituted liquid can be stored at -20ºC for 2 years. Centrifuge after first thaw to maximum product recovery. Aliquot into smaller volume to avoid repeated freeze-thaw cycles. Store aliquots at 4ºC for several days to 2 weeks.
50 µg, 100 µg