Source protein: Protein A affinity chromatography purified protein from mouse ascites fluid.
Clonality and Host Species: HA. C5, Mouse
Immunogen and Isotype: YPYDVPDYA (HA) synthetic peptide conjugated to KLH, IgG3
Specificity and reactivity: Detects the HA-tagged fusion proteins, regardless of the tag’s location in the fusion protein sequences (reacts with N-terminal, C-terminal or internal HA tagged fusion proteins)
Application: Western blot. Dot blot, ELISA, IHC/IS. Western blot (with ECL) 1-5 ng of HA-tagged fusion protein.
Available form: 50 µg of lyophilized antibody (lyophilized in 10mM PBS, pH 7.2 with 1% BSA and 0.05% Sodium azide; NaN3).
Working procedure: Western blot (with ECL) 1:1000-3,000 dilution incubates for 1 hour at room temperature. For the best results, determine the optimal working antibody dilution for your protein of interest.
Storage: Supplied lyophilized antibodies can be stored at -20ºC for 3 years. Reconstitute the lyophilized antibody with distilled water to 1mg/ml or a desired concentration before use. Reconstituted liquid can be stored at -20ºC for 2 years. Centrifuge after first thaw to maximum product recovery. Aliquot into smaller volume to avoid repeated freeze-thaw cycles. Store aliquots at 4ºC for several days to 2 weeks.
50 µg, 100 µg