Goat a-Mouse IgG, Alkaline phosphatase (AP) Labeled (1.0mg/ml)

$324.00

Cat. No.: 10019 Categories: ,

Description

Product name: Goat α-Mouse IgG, Alkaline phosphatase (AP) Labeled

Host species: Goat

Target species: Mouse

Specificity: By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.

Tested applications: Suitable for Western Blot, ELISA, ICC, IHC-P

Conjugation: Alkaline Phosphatase

Form: Liquid

Storage: Shipped at 4°C. Upon receiving, store at +4°C.

Storage buffer: 0.2% BSA, 0.1M Sodium chloride, 50mM HEPES, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 7.1

Preservative: 0.1% Sodium Azide

Concentration: 1 mg/ml 

Purity: Immunogen affinity purified, using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP).

Molar enzyme: antibody protein 1:1

Clonality: Polyclonal

Isotype: IgG


Recommended Dilutions: Below are the recommended starting dilutions (optimal dilutions/concentrations) and should be determined by the end user:

Application

Recommended Dilution

Western Blot

1:2000 – 1:20000
Chemiluminescent detection

1:5000 – 1:30000
Colorimetric detection

ELISA

1:10000 – 1:100000

Figure: Western blot – Developed, using Goat α-Mouse IgG, Alkaline phosphatase (AP) Labeled antibody
All lanes: Anti-beta Actin antibody, Loading Control at 1 µg/ml
Lanes 1 -6: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate proteins at 10 µg per lane
Secondary
Lanes 1 – 2: Goat α-Mouse IgG, Alkaline phosphatase (AP) Labeled antibody at 1:5000 dilution
Lanes 3 – 4: Goat α-Mouse IgG, Alkaline phosphatase (AP) Labeled antibody at 1:20000 dilution
Lanes 5 – 6: Goat α-Mouse IgG, Alkaline phosphatase (AP) Labeled antibody at 1:50000 dilution
Developed using the ECL technique with Exposure time 4 minutes

Additional information

Size

1.0ml

Certificate of Analysis