Protein A affinity
chromatography purified protein from mouse ascites fluid.
Clonality and Host Species:
Immunogen and Isotype:
RFP from the Discosoma
sp. (FLAG) synthetic N-terminal
peptide conjugated to KLH, IgG1
Specificity and reactivity:
Detects the native and
denatured forms of RFP and its variants such as TagRFP, Turbo RFP ds RED
mCherry, mOrange .
Western blot, Dot blot,
ELISA, IHC/IS, Western blot (with ECL) 1-10 ng of RFP or RFP fusion protein.
Lyophilized 1mg/mL (lyophilized
in 10mM PBS, pH 7.2 with 1% BSA and 0.05% Sodium azide; NaN3)
Western blot (with ECL)
1:1000-3,000 dilution incubate for 1 hour at room temperature. For the best
results, determine the optimal working antibody dilution for your protein of
antibodies can be stored at 200C for 3 years.
lyophilized antibody with distilled water to 1mg/ml or a desired concentration
Reconstituted liquid can be
stored at -20oC for 2 years. Centrifuge after first thaw to maximum
product recovery. Aliquot into smaller volume to avoid repeated freeze-thaw
cycles. Store aliquots at 40C
for several days to 2 weeks.
50 µg, 100 µg