Goat a-Rabbit IgG, Alkaline phosphatase (AP) Labeled (1mg/ml)

$299.00

Cat. No.: 10020 Categories: ,

Description

Product name: Goat α-Rabbit IgG, Alkaline phosphatase (AP) Labeled (1mg/ml)

Host species: Goat

Target species: Rabbit

Conjugation: Alkaline Phosphatase

Tested applications: ICC, IHC-P, ELISA, WB 

Form: Liquid

Concentration: 1 mg/ml 

Purity: Immunogen affinity purified

Purification notes: This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP).

Clonality: Polyclonal

Isotype: IgG

Storage buffer: Constituents: 0.2% BSA, 0.1M Sodium chloride, 50mM HEPES, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 7.1 

Preservative: 0.1% Sodium Azide

Molar enzyme: Ab protein 1:1

Storage Condition: Shipped at 4°C. Store at +4°C.

Recommended Dilutions: Below are the recommended starting dilutions; optimal dilutions/concentrations and should be determined by the end user:

Application

Recommended Dilution

Western Blot

1:5000 – 1:50000 Chemiluminescent detection

ELISA

1:5000 – 1:50000

IHC-P

1:100 – 1:1000

ICC

Use at an assay dependent dilution

Western blot – Secondary Antibody: Goat
α-Rabbit IgG Alkaline phosphatase (AP) Labeled

All lanes: Anti-beta Actin antibody –
Loading Control at 1 µg/ml

Lanes 1-6: HeLa (Human epithelial
carcinoma cell line) Whole Cell Lysate, 10 µg protein per lane.

 

Secondary

Lanes 1 – 2: Goat polyclonal Secondary
Antibody to Rabbit IgG – H&L (AP) 
at  1/5000 dilution

Lanes 3 – 4: Goat polyclonal Secondary
Antibody to Rabbit IgG – H&L (AP)  at
1/20000 dilution

Lanes 5 – 6: Goat polyclonal Secondary
Antibody to Rabbit IgG – H&L (AP)  at
1/50000 dilution

 

Developed
using the ECL technique

Exposure
time:
3
minutes

Additional information

Size

1.0ml

Certificate of Analysis