Protein A affinity
chromatography purified protein from mouse ascites fluid.
Clonality and Host Species:
Immunogen and Isotype:
Recombinant full length
ERK1 protein, IgG1
Specificity and reactivity:
Detects the ERK1 and
ERK2 protein (e.g. from human and mouse cells).
IP, Western blot
Lyophilized 1mg/mL (lyophilized
in 10mM PBS, pH 7.2 with 1% BSA and 0.05% Sodium azide; NaN3)
Western blot (with ECL)
1:1000 dilution incubates for 1 hour at room temperature. For the best results,
determine the optimal working antibody dilution for your protein of interest.
antibodies can be stored at -200C for 3 years.
lyophilized antibody with distilled water to 1mg/ml or a desired concentration
Reconstituted liquid can be
stored at -20oC for 2 years. Centrifuge after first thaw to maximum
product recovery. Aliquot into smaller volume to avoid repeated freeze-thaw
cycles. Store aliquots at 40C
for several days to 2 weeks.
50 µg, 100 µg