Goat a-Rat IgG, Alkaline phosphatase (AP) Labeled (1mg/ml)

$289.00

Cat. No.: 10021 Categories: ,

Description

Product name: Goat α-Rat IgG, Alkaline phosphatase (AP) Labeled (1mg/ml)

Host species: Goat

Target species: Rat

Conjugation: Alkaline Phosphatase

Tested applications: Western Blot, ELISA, IHC-P, ICC

Form: Liquid

Concentration: 1 mg/ml 

Purity: Immunogen affinity purified

Purification notes: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads.

Clonality: Polyclonal

Isotype: IgG

Storage buffer: Constituents: 0.878% Sodium chloride, 1% BSA, 0.788% Tris HCl, 0.0095% Magnesium chloride, 0.014% Zinc chloride, 50% Glycerol

Preservative: 0.1% Sodium Azide

Molar enzyme: Ab protein 1:1

Storage Condition: Shipped at 4°C. Store at +4°C.

Recommended Dilutions: Below are the recommended starting dilutions; optimal dilutions/concentrations and should be determined by the end user:

Application

Recommended Dilution

Western Blot

1:2000 – 1:20000 Chemiluminescent detection

ELISA

1:15000 – 1:20000

IHC-P

1:200 – 1:1000

ICC

Use at an assay
dependent dilution

Western blot – Goat α-Rat IgG, Alkaline phosphatase (AP) Labeled
All lanes : Anti-Tubulin antibody – Loading Control at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg per lane.
Secondary
Lanes 1 – 2: Goat polyclonal Secondary Antibody to Rat IgG – AP at 1:5000 dilution
Lanes 3 – 4: Goat polyclonal Secondary Antibody to Rat IgG – AP at 1:20000 dilution
Lanes 5 – 6: Goat polyclonal Secondary Antibody to Rat IgG – AP at 1:50000 dilution
Developed using the ECL technique
Exposure time: 5 minutes

Additional information

Size

1.0ml

Certificate of Analysis