Description
Agarose Type D-1 LE Low Electroendosmosis is specifically prepared for the use in electrophoretic studies to analyze DNA fragments from PCR products and digestion by restriction endonucleases, among others. Obtained from seaweeds, mostly Gelidium spp., agarose is a polymer composed of several agarobiose units that aggregate into a network with varying pore sizes. This property is used to separate nucleic acid fragments based on their lengths. Agarose is a gel at room temperature and remains firm at temperatures up to 65 ºC. In order to gelify, agarose must be dissolved, heated to its melting temperature (85-91ºC) and cooled down to 34-37 ºC to solidify as gel.
Features
- Extraordinary mechanical resistance for more reliable and easier handling.
- The possibility of varying pore size in accordance with particle size by modifying the gel concentration.
- Easy preparation of the gel by simple dilution in aqueous buffers either by standard boiling or microwaving.
- Greater thermal stability, due to high hysteresis (difference between gelling and melting temperatures).
- Excellent transparency of the gel & visibility.
- Exceptionally low absorption of staining agents.
- Absence of toxicity (the alternative is polyacrylamide which can be toxic)
Applications
- High electrophoresis mobility
- Nucleic acid analytical and preparatory electrophoresis
- Blotting
- Protein electrophoresis such as radial immunodiffusion
Additional information
| Size | 125 g, 250 g, 500 g |
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