The Fish Estradiol (E2) ELISA Kit assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for E2 and Horseradish Peroxidase (HRP) conjugated E2. The competitive inhibition reaction is launched between with HRP labeled E2 and unlabeled E2 with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of E2 in the sample. The color development is stopped and the intensity of the color is measured.
Product Type:Â ELISA Kit
Species: Fish
Sample Type: serum, plasma, cell culture supernatant, tissue homogenate.
Detect Range: 40 pg/ml-1000 pg/ml
Sensitivity: 40 pg/ml
Antigen Name: Estradiol(E2)
Abbreviation: E2
Protein Biological Process 1: Sex hormone
Assay Time: 1-5h
Sample Volume: 50-100ul
Detection Wavelength: 450 nm
Research Topic: Signal Transduction