HDAC8 Antibody, FITC conjugated

$210.00$377.00

Cat. No.: 42271 Categories: ,

Description

Aliases

HDAC8, HDACL1, CDA07

Antibody Type

Polyclonal Antibody

Species

Human

Uniprot ID

Q9BY41

Immunogen

Recombinant human Histone deacetylase 8 protein (1-377AA)

Raised In

Rabbit

Species Reactivity

Human

Tested Applications

ELISA;Not yet tested in other applications.

Background / Function

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Also involved in the deacetylation of cohesin complex protein SMC3 regulating release of cohesin complexes from chromatin. May play a role in smooth muscle cell contractility.

Isotype

IgG

Conjugate

FITC

Storage Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Caprylic Acid Ammonium Sulfate Precipitation purified

Literature

[1]”Structural studies of human histone deacetylase 8 and its site-specific variants complexed with substrate and inhibitors.” Dowling D.P., Gantt S.L., Gattis S.G., Fierke C.A., Christianson D.W.Biochemistry 47:13554-13563(2008) . [2]”Substrate binding to histone deacetylases as shown by the crystal structure of the HDAC8-substrate complex.” Vannini A., Volpari C., Gallinari P., Jones P., Mattu M., Carfi A., De Francesco R., Steinkuehler C., Di Marco S. EMBO Rep. 8:879-884(2007). [3]”Structural snapshots of human HDAC8 provide insights into the class I histone deacetylases.” Somoza J.R., Skene R.J., Katz B.A., Mol C., Ho J.D., Jennings A.J., Luong C., Arvai A., Buggy J.J., Chi E., Tang J., Sang B.-C., Verner E., Wynands R., Leahy E.M., Dougan D.R., Snell G., Navre M. expand/collapse author list Tari L.W. Structure 12:1325-1334(2004).

Additional information

Size

50μg, 100μg

Certificate of Analysis