Description
Aliases
H2A.X; H2AFX; H2a/x; HIST5-2AX;
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: P16104
NCBI Protein: NP_002096.1
Immunogen
Peptide sequence around phosphorylation site of serine 139 (Q-A-S(p)-Q-E) derived from Human Histone H2A.X.
Raised In
Rabbit
Species Reactivity
Human
Tested Applications
WB IF Recommended dilution: Predicted MW: 15kd, Western blotting: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Background / Function
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation. Yaneva M, et al. (2005) Nucleic Acids Res. 33(16): 5320-5330. Tsukuda T, et al.(2006) Nature. Author manuscript; available in PMC 2006 March 6.
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Ser139
Additional information
| Size | 100?l |
|---|
