Product Type: ELISA Kit
Species:Â Human
Research Topic: Immunology
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernatant and other biological fluids
Detection Range: 0.63 ng/ml -40 ng/mL
Sensitivity: 0.254 ng/mL
Alternative Names: Adipsin ELISA Kit; Adipsin/complement factor D ELISA Kit; Adn ELISA Kit; C3 convertase activator ELISA Kit; CFAD_HUMAN ELISA Kit; CFD ELISA Kit; Complement factor D ELISA Kit; complement factor D preproprotein ELISA Kit; D component of complement ELISA Kit; DF ELISA Kit; FactorD ELISA Kit; PFD ELISA Kit; Properdin factor D ELISA Kit
Antigen Name: Complement Factor D (Adipsin)
Abbreviation: CFD
Protein Biological Process 1: Complement
Protein Biological Process 3: Complement alternate pathway
Assay Time: 3.5 h
Sample Volume: 50-100 ul
Detection Wavelength: 450 nm
Test Principle: The test principle applied in this ELISA kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit is pre-coated with an antibody specific to Human CFD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CFD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated.
After the incubation, the TMB substrate solution is added and only those wells that contain Human CFD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the reaction color is measured spectrophotometrically at 450nm ± 10nm. The concentration of Human CFD in the samples is then determined by comparing the OD of the samples to the standard curve.
SAMPLE Standard Curve:
