Our Human Testosterone ELISA Kit is an excellent kit for quantitative determination of human testosterone concentrations in serum, plasma, saliva and other biological fluids.
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Testosterone. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Testosterone. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Testosterone in the samples is then determined by comparing the OD of the samples to the standard curve.
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Detectable Sample Type: serum, plasma, saliva, urine and other biological fluids
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Sensitivity: 0.67 ng/mL
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Detection Range: 1.56-100 ng/mL
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Specificity: This assay has high sensitivity and excellent specificity for detection of Human Testosterone. No significant cross-reactivity or interference between Human Testosterone and analogues was observed.
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Shelf Life: Refer to the outer kit label for the specific shelf life.
