Description
Aliases
SRK, STD, TZK, STCD, ZAP-70
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: P43403
NCBI Protein: NP_001070.2
Immunogen
Peptide sequence around phosphorylation site of tyrosine 319 (S-P-Y(p)-S-D) derived from Human Zap-70.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB IHC IF Recommended dilution: Predicted MW: 70kd, Western blotting: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Background / Function
Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not. Salomon AR, et al. (2003) Proc Natl Acad Sci U S A; 100(2): 443-448 Ku GM, et al. (2001) EMBO J; 20(3): 457-465 Tang J, et al. (1999) Proc Natl Acad Sci U S A; 96(17): 9775-9780 Zhao Q, et al. (1996) Mol Cell Biol; 16(12): 6765-6774 Williams BL, et al. (1999) EMBO J; 18(7): 1832-1844 Adjali O, et al. (2005) J Clin Invest; 115(8): 2287-2295.
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Tyr319
Additional information
| Size | 100?l |
|---|
