Description
Aliases
PKM, PK3, OIP3, PK2
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot#: P14618 NCBI Protein#: NP_872270.1
Immunogen
Peptide sequence around phosphorylation site of serine 37(I-D-S(p)-P-P) derived from Human PKM2.
Raised In
Rabbit
Species Reactivity
Human
Tested Applications
WB IF Recommended dilution: Predicted MW: 60kdWestern Blot: 1:500~1000
Background / Function
Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.Weiwei Yang1, Yanhua Zheng1, Yan Xia1, Haitao Ji, "ERK1/2-dependent phosphorylation and nucleartranslocation of PKM2 promotes the Warburg effect." Nature Cell Biology(2012)|doi:10.1038/ncb2629Received16 August 2012 Accepted24 October 2012 Published online25 November 2012
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Ser37
Additional information
Size | 100?l |
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