ADDA; Erythrocyte adducin alpha subunit;
NCBI Protein: NP_001110.2
Peptide sequence around phosphorylation site of serine 726 (T-P-S(p)-F-L) derived from Human ADD1.
Human Mouse Rat
WB IHC IF Recommended dilution: Predicted MW: 130kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background / Function
Adducins are a family of cytoskeleton proteins encoded by three genes (a, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. a- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. a- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described. Pariser H, et al. Proc Natl Acad Sci USA 2005 Aug 30; 102(35): 12407-12Tamaru S, et al. Biochem Biophys Res Commun 2005 Jul 01; 332(2): 347-51Barkalow KL, et al. J Cell Biol 2003 May 12; 161(3): 557-70
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.