ARF6, ADP-ribosylation factor 6
Recombinant human ADP-ribosylation factor 6 protein (1-175AA)
ELISA;Not yet tested in other applications.
Background / Function
GTP-binding protein involved in protein trafficking that regulates endocytic recycling and cytoskeleton remodeling. Required for normal completion of mitotic cytokinesis. Plays a role in the reorganization of the actin cytoskeleton and the formation of stress fibers. May also modulate vesicle budding and uncoating within the Golgi apparatus. Involved in the regulation of dendritic spine development, contributing to the regulation of dendritic branching and filopodia extension. Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase.
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Caprylic Acid Ammonium Sulfate Precipitation purified
Literature”The assembly of a GTPase-kinase signalling complex by a bacterial catalytic scaffold.” Sel unin A.S., Sutton S.E., Weigele B.A., Reddick L.E., Orchard R.C., Bresson S.M., Tomchick D.R., Alto N.M. Nature 469:107-111(2011). ”The structure of an Arf-ArfGAP complex reveals a Ca2+ regulatory mechanism.” Ismail S.A., Vetter I.R., Sot B., Wittinghofer A. Cell 141:812-821(2010). ”The structural basis of Arf effector specificity: the crystal structure of ARF6 in a complex with JIP4.” Isabet T., Montagnac G., Regazzoni K., Raynal B., El Khadali F., England P., Franco M., Chavrier P., Houdusse A., Menetrey J. EMBO J. 28:2835-2845(2009).