Atp5b Antibody, HRP conjugated

$210.00$377.00

Description

Aliases

ATP synthase subunit beta, mitochondrial, Atp5b

Antibody Type

Polyclonal Antibody

Species

Mouse

Uniprot ID

P56480

Immunogen

Recombinant mouse ATP synthase subunit beta, mitochondrial protein (47-529AA)

Raised In

Rabbit

Species Reactivity

mouse

Tested Applications

ELISA;Not yet tested in other applications.

Background / Function

Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 – containing the extramembraneous catalytic core, and F0 – containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F1. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.

Isotype

IgG

Conjugate

HRP

Storage Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Caprylic Acid Ammonium Sulfate Precipitation purified

Research

Others

Literature

[1]”Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.”Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R.,

Additional information

Size

50μg, 100μg

Certificate of Analysis