RECQ2; RECQL3; type 2; EC 3.6.1; RecQ protein-like 3
NCBI Gene#: 641;
Peptide sequence around phosphorylation site of threonine 99 (Q-E-T(p)-Q-R) derived from Human Bloom Syndrome.
WB IHC Recommended dilution: Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background / Function
Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3′-5′ direction. Involved in 5′-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5′-ssDNA. Negatively regulates sister chromatid exchange (SCE).Ellis N.A., Cell 83:655-666(1995).Karow J.K., J. Biol. Chem. 272:30611-30614(1997).Pichierri P., EMBO J. 23:3154-3163(2004).
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.