EPHA2/3/4 (Phospho-Tyr588/596) Antibody

$454.00

Cat. No.: 38902 Categories: ,

Description

Aliases

ECK; MPK-5; SEK2; kinase EphA2;

Antibody Type

Polyclonal Antibody

Uniprot ID

Swiss-Prot#: P29317/P29320/P54764;
NCBI Gene#

Immunogen

Peptide sequence around phosphorylation site of tyrosine 588/596 (K-T-Y(p)-V-R)/(R-T-Y(p)-V-D) derived from Human EPHA2/3/4.

Raised In

Rabbit

Species Reactivity

Human Mouse

Tested Applications

WB IF Recommended dilution: Western blotting: 1:500~1:1000, Immunofluorescence: 1:100~1:200

Background / Function

Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway. Lindberg R.A., Mol. Cell. Biol. 10:6316-6324(1990).Zhang Y., Mol. Cell. Proteomics 4:1240-1250(2005).Zhuang G., J. Biol. Chem. 282:2683-2694(2007)

Conjugate

Unconjugated

Storage Buffer

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Modification

Phospho-Tyr588/596

Additional information

Size

100?l

Certificate of Analysis