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Protein Electrophoresis

Protein Electrophoresis Electrophoresis is a simple yet effective tool that is used to separate proteins, nucleic acids, and other biological molecules in sample solutions. In proteomics, this technique is used to separate individual protein components in a sample by passing…

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Quantifying Proteins

  Quantifying protein concentrations is necessary to understand the total protein content in a sample and is important for normalizing different protein samples for subsequent applications such as western blotting or immunoprecipitation. This is because assays such as these require…

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What are Cloning Vectors?

In our last blog, we looked at genomic library construction and gene cloning. One important aspect of gene cloning is deciding what cloning vector you are going to use to create recombinant DNA. There are a variety of cloning vectors…

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Genomic Libraries: Construction and Applications

DNA sequencing became possible in the late 1970s and has been one of the most influential developments in biomedical research to date. This technology has rapidly expanded since the publication of the human genome in 2003 and genomics is now…

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Measuring Apoptosis

ELISAs – what are they and how do they work?

An Enzyme-Linked Immuno-Sorbent Assay (ELISA) is a highly sensitive immunoassay used to detect and quantify antibodies, hormones, peptides, and proteins. This plate-based assay has a typical detection range of 0.1 to 1 fmole or 0.01 ng to 0.1 ng, making…

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