DL-Luciferin; 1-(4,5-dimethoxy-2-nitrophenyl) ethyl ester

Luciferin is a common bioluminescent reporter used for in vivo imaging of the expression of the luciferase. This substrate for the Firefly luciferase enzyme utilizes ATP and Mg2+ as co-factors and emits a characteristic yellow-green emission in the presence of oxygen, which shifts to red light in vivo at 37°C. DL is a racemic mixture of D-Luciferin and L-Luciferin.

DMNPE-caged luciferin can be used to measure intracellular functions since it readily crosses cell membranes. Once it is inside the cell, active luciferin can be released by UV light or by the action of endogenous intracellular esterases. DMNPE-caged luciferin makes it easier to follow the changes in gene expression in live cells. It can also be used in combination with DMNPE-caged ATP.

CAS Number:                  154140-55-3

Molecular Formula:       C21H19N3O7S2

Molecular Weight:          489.517 g/mol

InChI Key:                         KQVRVPOUBLPROI-VXPUYCOJSA-N