Name: DMEM/F-12 GlutaPlus No HEPES
SKU: G4610
Availability: InStock

DMEM/F-12 GlutaPlus No HEPES

$46.00 – $324.00

Size

500 mL, 2×500 mL, 4×500 mL, 6×500 mL, 10×500 mL

Product Description

DMEM/F-12 medium was modified by mixing DMEM with Ham’s F-12 medium at a ratio of1:1. The modified DMEM/F-12 medium is richer in nutrients and contains more micronutrients, which is a widely used basal medium for supporting the growth of many different mammalian cells, as well as for the culture of mammalian cells at low serum levels and for clonal density culture. DMEM/F-12 has also been used as a base medium for the development of serum-free medium.

This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, without protein or growth factors. Add 5-10% serum or serum-free additives depending on the cell type.

This product is sterilized by 0.1μm filter membrane, pH 7.0-7.4, contains 17.5 mM D-glucose,2.5 mML-Alanyl-L-Glutamine,0.5 mM sodium pyruvate, phenol red indicator, no HEPES.

Storage and Shipping Conditions

Ship at room temperature; Store away from light at 2-8℃, valid for 12 months.

Note

1. This product has been filtered and sterilized. Attention should be paid to aseptic operation to avoid contamination.

2. For best results, do not freeze and thaw repeatedly.

3. For your safety and health, please wear safety glasses, gloves and protective clothing.

For Research Use Only!

G4610DMEM/F-12Formula

Components	Concentration		Components	Concentration
Components	mg/L	mM	Components	mg/L	mM
Amino acids			Inorganic salts
Glycine	18.75	0.250	Calcium Chloride (CaCl₂)(anhyd.)	116.6	1.050
L-Alanine	4.45	0.050	Cupric Sulfate (CuSO₄·5H₂O)	0.0013	0.0000052
L-Arginine monohydrochloride	147.5	0.699	Ferric Nitrate (Fe(NO₃)₃·9H₂O)	0.05	0.0001238
L-Asparagine monohydrate	7.5	0.050	Ferrous Sulfate (FeSO₄·7H₂O)	0.417	0.0015
L-Asparagine	6.65	0.050	Magnesium chloride (MgCl₂)(anhyd.)	28.64	0.301
L-Cysteine hydrochloride hydrate	17.56	0.100	Magnesium sulfate (MgSO₄) (anhyd.)	48.84	0.407
L-Cystine dihydrochloride	31.29	0.100	Potassium Chloride (KCl)	311.8	4.157
L-Glutamic acid	7.35	0.050	Sodium bicarbonate(NaHCO₃)	2438	29.02
L-Alanyl-L-Glutamine	543.0	2.500	Sodium chloride (NaCl)	6995.5	120.6
L-Histidine hydrochloride monohydrate	31.48	0.150	Disodium (hydrogen) phosphate(Na₂HPO₄)(anhyd.)	71.02	0.500
L-Isoleucine	54.47	0.416	Sodium dihydrogen phosphate(NaH₂PO₄)(anhyd.)	54.34	0.453
L-Leucine	59.05	0.451	Zinc Sulfate(ZnSO₄·7H₂O)	0.432	0.0015
L-Lysine hydrochloride	91.25	0.499	Other components
L-Methionine	17.24	0.116	D-Glucose (Dextrose)	3151	17.51
L-Phenylalanine	35.48	0.215	Hypoxanthine Na	2.39	0.015
L-Proline	17.25	0.150	Linoleic Acid	0.042	0.00015
L-Serine	26.25	0.250	Lipoic acid	0.105	0.00051
L-Threonine	53.45	0.449	Phenol Red	8.1	0.022
L-Tryptophan	9.02	0.044	Putrescine dihydrochloride	0.081	0.00050
L-Tyrosine disodium salt	55.79	0.214	Thymidine	0.365	0.00151
L-Valine	52.85	0.452	Sodium pyruvate	55	0.500
Vitamins			HEPES	–	–
Biotin	0.0035	0.0000143
Choline chloride	8.98	0.06414
D-Calcium pantothenate	2.24	0.00470
Folic Acid	2.65	0.00601
Niacinamide	2.02	0.01656
Pyridoxine hydrochloride	2.013	0.00977
Riboflavin	0.219	0.00058
Thiamine hydrochloride	2.17	0.00644
Vitamin B12	0.68	0.00050
i-Inositol	12.6	0.07

MAECENAS IACULIS

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ADIPISCING CONVALLIS BULUM

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-29%

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Size	500 mL, 2×500 mL, 4×500 mL, 6×500 mL, 10×500 mL

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SKU: G4610 Categories: Culture Media, Mammalian Cell Culture

Description

Components	Concentration		Components	Concentration
Components	mg/L	mM	Components	mg/L	mM
Amino acids			Inorganic salts
Glycine	18.75	0.250	Calcium Chloride (CaCl₂)(anhyd.)	116.6	1.050
L-Alanine	4.45	0.050	Cupric Sulfate (CuSO₄·5H₂O)	0.0013	0.0000052
L-Arginine monohydrochloride	147.5	0.699	Ferric Nitrate (Fe(NO₃)₃·9H₂O)	0.05	0.0001238
L-Asparagine monohydrate	7.5	0.050	Ferrous Sulfate (FeSO₄·7H₂O)	0.417	0.0015
L-Asparagine	6.65	0.050	Magnesium chloride (MgCl₂)(anhyd.)	28.64	0.301
L-Cysteine hydrochloride hydrate	17.56	0.100	Magnesium sulfate (MgSO₄) (anhyd.)	48.84	0.407
L-Cystine dihydrochloride	31.29	0.100	Potassium Chloride (KCl)	311.8	4.157
L-Glutamic acid	7.35	0.050	Sodium bicarbonate(NaHCO₃)	2438	29.02
L-Alanyl-L-Glutamine	543.0	2.500	Sodium chloride (NaCl)	6995.5	120.6
L-Histidine hydrochloride monohydrate	31.48	0.150	Disodium (hydrogen) phosphate(Na₂HPO₄)(anhyd.)	71.02	0.500
L-Isoleucine	54.47	0.416	Sodium dihydrogen phosphate(NaH₂PO₄)(anhyd.)	54.34	0.453
L-Leucine	59.05	0.451	Zinc Sulfate(ZnSO₄·7H₂O)	0.432	0.0015
L-Lysine hydrochloride	91.25	0.499	Other components
L-Methionine	17.24	0.116	D-Glucose (Dextrose)	3151	17.51
L-Phenylalanine	35.48	0.215	Hypoxanthine Na	2.39	0.015
L-Proline	17.25	0.150	Linoleic Acid	0.042	0.00015
L-Serine	26.25	0.250	Lipoic acid	0.105	0.00051
L-Threonine	53.45	0.449	Phenol Red	8.1	0.022
L-Tryptophan	9.02	0.044	Putrescine dihydrochloride	0.081	0.00050
L-Tyrosine disodium salt	55.79	0.214	Thymidine	0.365	0.00151
L-Valine	52.85	0.452	Sodium pyruvate	55	0.500
Vitamins			HEPES	–	–
Biotin	0.0035	0.0000143
Choline chloride	8.98	0.06414
D-Calcium pantothenate	2.24	0.00470
Folic Acid	2.65	0.00601
Niacinamide	2.02	0.01656
Pyridoxine hydrochloride	2.013	0.00977
Riboflavin	0.219	0.00058
Thiamine hydrochloride	2.17	0.00644
Vitamin B12	0.68	0.00050
i-Inositol	12.6	0.07

Manual

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Safety Datasheet

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Certificate of Analysis

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