HDAC2(Phospho-Ser394) Antibody

$454.00

Cat. No.: 39095 Categories: ,

Description

Aliases

HD2

Antibody Type

Polyclonal Antibody

Uniprot ID

Swiss-Prot: Q92769
NCBI Protein: NP_001518.2

Immunogen

Peptide sequence around phosphorylation site of serine 394 (E-D-S(p)-G-D) derived from Human HDAC2.

Raised In

Rabbit

Species Reactivity

Human Mouse Rat

Tested Applications

WB IHC IF Recommended dilution: Predicted MW: 60kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Background / Function

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes Tsai SC, et al.(2002)J Biol Chem; 277(35): 31826-33

Conjugate

Unconjugated

Storage Buffer

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Modification

Phospho-Ser394

Additional information

Size

100?l

Certificate of Analysis