APRF; Acute-phase response factor; HIES
NCBI Protein: NP_003141.2
Peptide sequence around phosphorylation site of tyrosine 705 (A-P-Y(p)-L-K) derived from Human STAT3.
Human Mouse Rat
WB IHC IF Recommended dilution: Predicted MW: 88kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background / Function
Transcription factor that binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. Fan G, et al. (2003) J Biol Chem. 278(52): 52432-52436. Barry FA, et al. (2003) FEBS Lett. 553(1-2): 173-178. Welsh, et al. (1996) Trends Cell Biol. 6: 274-279. Srivastava A K, et al. (1998) Mol Cell Biochem. 182: 135-141.
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.