Source protein: Protein A affinity purified protein from mouse ascites fluid
Clonality and Host Species: BA3R, Mouse
Immunogen and Isotype: Beta actin N-terminal peptide-KLH conjugates, IgG2b
Specificity and reactivity: Detects native and denatured forms of beta actin (~42 KDa) from rat, mouse, rabbit, chicken and human
Application: Western blot, Dot blot, ELISA, IHC/IS
Available form: 50 µg of lyophilized antibody (lyophilized in 10mM PBS, pH 7.2 with 1% BSA and 0.05% Sodium azide; NaN3)
Working procedure: Western blot (with ECL) 1:1000 – 5,000 dilution incubates for 1 hour at room temperature. For the best results, determine optimal working antibody dilution for your protein of interest.
Storage: Supplied lyophilized antibodies can be stored at -20ºC for 3 years. Reconstitute the lyophilized antibody with distilled water to 1mg/ml or a desired concentration before use. Reconstituted liquid can be stored at -20ºC for 2 years. Centrifuge after first thaw to maximum product recovery. Aliquot into smaller volume to avoid repeated freeze-thaw cycles. Store aliquots at 4ºC for several days to 2 weeks.
50 µg, 100 µg, 200 µg