HER2(Phospho-Tyr1221/Tyr1222) Antibody

$454.00

Cat. No.: 38987 Categories: ,

Description

Aliases

C-erbB-2; ErbB2;

Antibody Type

Polyclonal Antibody

Uniprot ID

Swiss-Prot: P04626
NCBI Protein: NP_001005862.

Immunogen

Peptide sequence around phosphorylation site of tyrosine1221/1222 (N-L-Y(p)-Y(p)-W) derived from Human HER2.

Raised In

Rabbit

Species Reactivity

Human

Tested Applications

WB IF Recommended dilution: Predicted MW: 185kd, Western blotting: 1:500~1:1000, Immunofluorescence: 1:100~1:200

Background / Function

Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF-a and amphiregulin. Marone R, et al. (2004) Nat Cell Biol; 6(6): 515-22. Ren Z, et al. (2002) J Biol Chem; 277(41): 38486-93.

Conjugate

Unconjugated

Storage Buffer

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Modification

Phospho-Tyr1221/Tyr1222

Additional information

Size

100?l

Certificate of Analysis