hnRNP K (Phospho-Ser216) Antibody

$454.00

Cat. No.: 38695 Categories: ,

Description

Aliases

Heterogeneous nuclear ribonucleoprotein K;hnRNP K;Transformation up-regulated nuclear protein; TUNP

Antibody Type

Polyclonal Antibody

Uniprot ID

Swiss-Prot#:P61978;
NCBI Gene#:3190

Immunogen

Peptide sequence around phosphorylation site of serine 216 (S-E-S(p)-P-I) derived from Human hnRNP K.

Raised In

Rabbit

Species Reactivity

Human Mouse Rat

Tested Applications

WB Recommended dilution: Western blotting: 1:500~1:3000,

Background / Function

One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. Plays an important role in p53/TP53 response to DNA damage, acting at the level of both transcription activation and repression. When sumoylated, acts as a transcriptional coactivator of p53/TP53, playing a role in p21/CDKN1A and 14-3-3 sigma/SFN induction By similarity. As far as transcription repression is concerned, acts by interacting with long intergenic RNA p21 (lincRNA-p21), a non-coding RNA induced by p53/TP53. This interaction is necessary for the induction of apoptosis, but not cell cycle arrest.

Conjugate

Unconjugated

Storage Buffer

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Modification

Phospho-Ser216

Additional information

Size

100?l

Certificate of Analysis