Our LDH-Cytotoxicity Fluorometric Assay Kit
offers a sensitive, rapid, and easy way for quantitatively measuring the lactate dehydrogenase (LDH), a
cytosolic enzyme that is released upon cell lysis or damage. The released LDH
from cells catalyzes the conversion of lactate to pyruvate and reduction of NAD+
to NADH and H+. Diaphorase, in presence of NADH catalyzes the reduction of non-fluorescent Resazurin to fluorescent Resarufin with excitation and emission at 535 and 587 nm respectively. The amount of fluorescence is directly proportional to the amount of LDH released from the damaged cells.