LDH Cytotoxicity Assay Kit, Fluorometric


Cat. No.: 10437 Category:


Our LDH-Cytotoxicity Fluorometric Assay Kit
offers a sensitive, rapid, and easy way for
quantitatively measuring the lactate dehydrogenase (LDH), a
cytosolic enzyme that is released upon cell lysis or damage. The released LDH
from cells catalyzes the conversion of lactate to pyruvate and reduction of NAD+
to NADH and H+. Diaphorase, in presence of NADH catalyzes the
reduction of non-fluorescent Resazurin to fluorescent Resarufin with excitation and emission at 535 and 587 nm respectively. The amount of
fluorescence is directly proportional to the amount of LDH released from the damaged cells.

Additional information


500 Assays