MACS; MARCS; PKCSL; PRKCSL; Protein kinase C substrate
NCBI Protein: NP_002347.5
Peptide sequence around phosphorylation site of serine 158 (R-F-S(p)-F-K) derived from Human MARCKS.
Human Mouse Rat
WB IF Recommended dilution: Predicted MW: 80kd, Western blotting: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Background / Function
MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.Pariser H, et al. Proc Natl Acad Sci U S A 2005 Aug 30; 102(35): 12407-12412Nagumo H, et al. Biochem Biophys Res Commun 2001 Jan 26; 280(3): 605-609Yamamoto H, et al. Arch Biochem Biophys 1998 Nov 15; 359(2): 151-159
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.