ERK activator kinase 2; MAP kinase kinase 2; MAP2K2; MAPK/ERK kinase 2; MAPKK 2
NCBI Protein: NP_109587.1
Peptide sequence around phosphorylation site of threonine 394 (P-G-T(p)-P-T) derived from Human MEK-2.
Human Mouse Rat
WB IHC IF Recommended dilution: Predicted MW: 44kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background / Function
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases. Crews C M, et al. (1992) Science. 258:478-480.Alessi D R, et al. (1994) EMBO J. 13:1610-1619.Rosen L B, et al. (1994) Neuron. 12:1207-1221.Cowley S, et al. (1994) Cell. 77:841-852.
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.