PKA a/ß CAT (Phospho-Thr197) Antibody

$454.00

Cat. No.: 39071 Categories: ,

Description

Aliases

PRKACA ; PRKACB; PKACA;

Antibody Type

Polyclonal Antibody

Uniprot ID

Swiss-Prot#: P17612;
NCBI Gene#: 5566;
N

Immunogen

Peptide sequence around phosphorylation site of threonine 197(R-T-W(p)-T-L) derived from Human PKA α/β CAT.

Raised In

Rabbit

Species Reactivity

Human Mouse Rat

Tested Applications

WB IHC IF Recommended dilution: Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Background / Function

Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA and VASP. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts. Ghislaine Garrel, Endocrinology, Jun 1997; 138: 2259. W Li, Learn. Mem., Jan 1996; 2: 320 – 333. Sharmin Schauble, J. Biol. Chem., May 2007; 282: 14952 – 14959. Yong G. Wang, J. Gen. Physiol., Jan 1998; 111: 113.

Conjugate

Unconjugated

Storage Buffer

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Modification

Phospho-Thr197

Additional information

Size

100?l

Certificate of Analysis