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Our Plasmid DNA Extraction Kit isolates high quality plasmid DNA from 1-5 mL E. coli bacterial cultures. Our kit is supplied with an enhanced DNA binding columns that produces high yields of plasmid. This quick and easy method adapted in our kit eliminates toxic phenol/chloroform extractions or lengthy ethanol precipitations. The plasmid DNA is extracted using our proprietary DNA matrix in a spin column format, which involves harvesting the bacteria followed by alkaline lysis and neutralization of the lysate, before passing through a column. When the neutralized lysate is passed through the column, the plasmid DNA is retained in the matrix by adsorption, which is then eluted either with TE Buffer or sterile deionized water. The plasmid DNA extracted with our kit is ready to use for downstream applications, such as restriction enzyme digestion, sequencing, subcloning and in vitro transcription. The plasmid yields are typically up to 20μg/prep with our kit.
| Items                | Cat. No. 10665-0
(50 Preps) |
Cat. No. 10665-1
(100 Preps) |
Cat. No. 10665-2
(200 Preps) |
| Suspension Buffer | 15 mL | 30 mL | 60 mL |
| Lysis Buffer | 25 mL | 50 mL | 100 mL |
| Neutralizing Buffer | 25 mL | 50 mL | 100 mL |
| DNA Wash Buffer* | 20 mL* | 2 x 20 mL* | 4 x 20 mL* |
| Plasmid Mini Columns with Collection Tube | 50 | 100 | 200 |
| SP-RNase | 0.5 mL | 0.5 mL | 0.5 mL |
| TE Buffer | 10 mL | 10 mL | 10 mL |
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