Our RIPA; Radio-Immunoprecipitation Assay Lysis Buffer is a complete cell lysis solution, used for rapid and efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells. The buffer is very effective in extracting cytoplasmic, nuclear and membrane proteins and the time for protein extraction takes about 60 minutes. The RIPA lysis buffer contains non-ionic and ionic detergents, that allows extraction of proteins from different cell types and membrane structures. The supplied RIPA buffer contains 1% NP-40, 1% sodium deoxycholate, 0.1% SDS with 25mM Tris and 150mM NaCl. RIPA buffer ensures efficient cell lysis and protein solubilization preventing protein degradation and interference with protein immunoreactivity and biological activity.
Since most antibodies and protein antigens are not adversely affected by the components of the RIPA Buffer, proteins extracted with this buffer are compatible with various downstream application, such as immunoprecipitation and molecular pull-down assays, including reporter assays, protein assays, immunoassays and protein purification. RIPA buffer minimizes non-specific protein-binding interactions and keep low background, while allowing most specific interactions to occur, enabling studies of relevant protein-protein interactions.
The RIPA Lysis Buffer is used for rapid, efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells. Our RIPA Lysis Buffer is highly effective and efficient for lysis and protein solubilization of wide range of samples, including mammalian cell and cover membranous, cytoplasmic, and nuclear protein extractions. It enables efficient cell lysis and protein solubilization while avoiding protein degradation and interference with the proteins′ immunoreactivity and biological activity. RIPA Buffer also results in low background in immunoprecipitation and molecular pull-down assays.
FEATURES:
- Ready to use solution
- Prepared with highest purity chemicals
- Â 0.22 um filter sterilized
| Name | Cat. No. 10456-0 | Cat. No. 10456-1 | Cat. No. 10456-2 | Storage Temp* |
| RIPA Lysis Buffer | 125 mL | 250 mL | 500 mL | 40C to RT |
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