Description

General Description

WST‐1 Cell Cytotoxicity Assay is a sensitive and reliable assay for cell cytotoxicity and proliferation. The assay principle is based upon the reduction of the tetrazolium salt WST‐1 to formazan by cellular dehydrogenases. The generation of the dark yellow colored formazan is measured at 420‐480nm (optimal at 440nm) and is directly correlate to cell number. The assay is very convenient for performing in a single tissue culture wells and requires no washing, harvesting or solubilization of cells.