Description
Aliases
H3/b, H3FB
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: P68431
NCBI Protein: NP_003521.2
Immunogen
Peptide sequence around phosphorylation site of serine 10 (R-K-S(p)-T-G) derived from Human Histone H3.1.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB IHC IF Recommended dilution: Predicted MW: 17kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background / Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Dai J, et al. (2005) Genes Dev 19(4): 472-488. Yih LH, et al. (2005) Carcinogenesis 26(1): 53-63.
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Ser10
Additional information
Size | 100μl |
---|