15-LOX-B, 15-lipoxygenase 2, 15-LOX-2, Arachidonate 15-lipoxygenase type II, Linoleate 13-lipoxygenase 15-LOb, ALOX15B
Recombinant human Arachidonate 15-lipoxygenase B protein (1-676AA)
ELISA;Not yet tested in other applications.
Background / Function
Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Converts arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid/(15S)-HPETE. Also acts on linoleic acid to produce 13-hydroxyoctadecadienoic acid/13-HPODE. Has no detectable 8S-lipoxygenase activity but reacts with (8S)-HPETE to produce (8S,15S)-diHPETE. May regulate progression through the cell cycle and cell proliferation. May also regulate cytokine secretion by macrophages and therefore play a role in the immune response. May also regulate macrophage differentiation into proatherogenic foam cells.
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Caprylic Acid Ammonium Sulfate Precipitation purified
LiteratureThe structure of human 15-lipoxygenase-2 with a substrate mimic.”Kobe M.J., Neau D.B., Mitchell C.E., Bartlett S.G., Newcomer M.E.J. Biol. Chem. 289:8562-8569(2014). Arachidonate 15-lipoxygenase type B knockdown leads to reduced lipid accumulation and inflammation in atherosclerosis.Magnusson L.U., Lundqvist A., Karlsson M.N., Skalen K., Levin M., Wiklund O., Boren J., Hulten L.M.PLoS ONE 7:E43142-E43142(2012).