ATF2(Phospho-Ser62 or 44) Antibody

$454.00

Cat. No.: 38732 Categories: ,

Description

Aliases

CREB2; CREBP1;

Antibody Type

Polyclonal Antibody

Uniprot ID

Swiss-Prot: P15336
NCBI Protein: NP_001871.2

Immunogen

Peptide sequence around phosphorylation site of serine 62 or 44 (N-D-S(p)-V-I) derived from Human ATF2.

Raised In

Rabbit

Species Reactivity

Human Mouse Rat

Tested Applications

WB IHC Recommended dilution: Predicted MW: 65-75 kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100,

Background / Function

Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5′-GTGACGT[AC][AG]-3′), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2-c-Jun complex.Sevilla A, et al. (2004) J Biol Chem. 279(26):27458-27465.Sakurai A, et al. (1991) Biochem Biophys Res Commun. 181(2): 629-635.Abdel-Hafiz H A, et al. (1992) Mol Endocrinol. 6: 2079-2089.Gupta S, et al. (1995) Science. 267: 389-393.Van Dam H, et al. (1995) EMBO J. 14(8): 1798-1811.

Conjugate

Unconjugated

Storage Buffer

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Modification

Phospho-Ser62 or 44

Additional information

Size

100μl

Certificate of Analysis