Description
Aliases
AH119; c-Jun; p39; Jun A; AP1
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot#: P05412;
NCBI Gene#: 3725;
N
Immunogen
Peptide sequence around phosphorylation site of Serine 63(L-T-S(p)-P-D) derived from Human c-Jun.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB IHC Recommended dilution: Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background / Function
Transcription factor that recognizes and binds to the enhancer heptamer motif 5′-TGA[CG]TCA-3′.Wei W, et al. (2005). Cancer Cell. 8(1): 25-33.Sevilla A, et al. (2004). Oncogene.23(55): 8950-8958.Li L, et al. (2004). J Biol Chem.279(6): 4058-4065.Hurd C, et al. (2002). Oncogene.21(14): 2154-2160.
Conjugate
Unconjugated
Storage Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Ser63
Additional information
Size | 100?l |
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