Caspase-1 Substrate (Ac-YVAD-AFC) [1mM], Fluorometric


Cat. No.: 10049 Categories: ,


Caspase-1 Substrate (Ac-YVAD-AFC) [1mM], Fluorometric


General description

This product is a ready-to-use fluorometric substrate for Caspase-1/ICE and related caspases that recognize the amino acid sequence YVAD. Caspase-1 and related caspase activity can be quantified by fluorescent detection of free AFC after cleaved from the peptide substrate YVAD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized, using a hand-held long-UV lamp.


Synonyms:                     Ac-YVAD-AFC, Caspase-1 Substrate, Fluorogenic

Peptide Sequence:       Ac-Tyr-Val-Ala-Asp-AFC

Appearance:                  Liquid

Formulation:                  DMSO (1 mM)

Molecular Formula:      C₃₃H₃₆F₃N₅O₁₀

Molecular Weight:         719.7

InChi Key:                       GWKJISJKNKIJNP-UHFFFAOYSA-N

PubChem CID:               44135090

Handling:                        Protect from light and moisture

Storage temp.:                -20°C


Additional information



Certificate of Analysis