Caspase-3, 7 Substrate (Ac-DEVD-AFC) [1mM], Fluorometric


Cat. No.: 10053 Categories: ,


Caspase-3, 7 Substrate (Ac-DEVD-AFC) [1mM], Fluorometric


General description

This product is a ready-to-use fluorometric substrate for Caspase-3/CPP32 (Km = 9.7 µM) and related caspases that recognize the amino acid sequence DEVD. The sequence DEVD is based on Caspase-3 cleavage site in poly (ADP-ribose) polymerase (PARP). CPP32 and related caspase activity can be quantified by fluorescent detection of free AFC after cleaved from the peptide substrate DEVD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or a multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp.


Synonyms:                     Ac-DEVD-AFC, Caspase-3 Substrate Fluorogenic

Peptide Sequence:       Ac-Asp-Glu-Val-Asp-AFC

Appearance:                  Liquid

Formulation:                  DMSO (1 mM)

Molecular Formula:      C₃₀H₃₃F₃N₅O₁₃

Molecular Weight:         729

Solubility:                        DMSO

InChi Key:                       GZDRODOYEFEHGG-NUDCOPPTSA-N

PubChem CID:               644234

Handling:                        Protect from light and moisture

Storage temp.:                -20°C


Additional information



Certificate of Analysis