Description
Caspase-3, 7 Substrate (Ac-DEVD-AFC) [1mM], Fluorometric
General description
This product is a ready-to-use fluorometric substrate for Caspase-3/CPP32 (Km = 9.7 µM) and related caspases that recognize the amino acid sequence DEVD. The sequence DEVD is based on Caspase-3 cleavage site in poly (ADP-ribose) polymerase (PARP). CPP32 and related caspase activity can be quantified by fluorescent detection of free AFC after cleaved from the peptide substrate DEVD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or a multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp.
Synonyms: Ac-DEVD-AFC, Caspase-3 Substrate Fluorogenic
Peptide Sequence: Ac-Asp-Glu-Val-Asp-AFC
Appearance: Liquid
Formulation: DMSO (1 mM)
Molecular Formula: C₃₀H₃₃F₃N₅O₁₃
Molecular Weight: 729
Solubility: DMSO
InChi Key: GZDRODOYEFEHGG-NUDCOPPTSA-N
PubChem CID: 644234
Handling: Protect from light and moisture
Storage temp.: -20°C
Additional information
| Size | 250µl |
|---|
