Caspase-8 Substrate (Ac-IETD-AFC) [1mM], Fluorometric

Description

Caspase-8 Substrate (Ac-IETD-AFC) [1mM], Fluorometric

General description

This product is a ready-to-use fluorometric substrate for FLICE/Caspase-8 and related caspases that recognize the amino acid sequence IETD. The sequence IETD is based on Caspase-8 cleavage site in CPP32/Caspase-3 proenzyme. FLICE and related caspase activity can be quantified by fluorescent detection of free AFC after cleavage from the peptide substrate IETD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp.

Synonyms:                       Ac-IETD-AFC, Caspase-8 Substrate, Fluorogenic

Peptide Sequence:         Ac-Ile-Glu-Thr-Asp-AFC

Appearance:                    Liquid

Formulation:                    DMSO (2 mM)

Molecular Formula:        C₃₁H₃₈F₃N₅O₁₂

Molecular Weight:          729.7

InChi Key:                         CGUOWXRXLQXOKO-FCQBACSVSA-N

PubChem CID:                 25108570

Handling:                          Protect from light and moisture

Storage temp.:                -20°C

Additional information