Caspase-9 Substrate (Ac-LEHD-AFC) [1mM], Fluorometric


Cat. No.: 10063 Categories: ,


Caspase-9 Substrate (Ac-LEHD-AFC) [1mM], Fluorometric


General description

This product is a ready-to-use fluorometric substrate for Caspase-9/Mch-6 and related caspases that recognize the amino acid sequence LEHD. Caspase activity can be quantified by fluorescent detection of free AFC after cleavage from the peptide substrate LEHD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized, using a hand-held long-UV lamp.


Synonyms:                       Ac-LEHD-AFC, Caspase-9 Substrate, Fluorogenic

Peptide Sequence:         Ac-Leu-Glu-His-Asp-AFC

Appearance:                    Liquid

Formulation:                    DMSO (1 mM)

Molecular Formula:        C₃₃H₃₈F₃N₇O₁₁

Molecular Weight:           765.7

InChi Key:                         HULKIXRFKRCRHD-UHFFFAOYSA-N

PubChem CID:                 44134612

Handling:                          Protect from light and moisture

Storage temp.:                 -20°C


Additional information



Certificate of Analysis